Stability : 6 months
Formulation : 20 mM potassium phosphate, pH 7.2, containing 100 mM sodium chloride
Protocatechuate dioxygenase (PCD) catalyzes the proximal extradiol ring cleavage of protocatechuate (PCA) with attendant incorporation of both atoms of oxygen from O2. The PCD holoenzyme consists of α (23 kDa) and β (26.5 kDa) subunits, which form a tetramer with four ferric ions for a total weight of ~200 kDa.2 Iron is essential for activity because the substrate, PCA, initially binds directly to the iron through both hydroxyl groups to form a chelate complex. PCD is an important component of bacterial metabolism of aromatic compounds found in the soil.
Purity : ≥90%
Storage : -80°C
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