Apoptotic Blebs Assay Kit

Stability : 6 months
Description :
Apoptotic Blebs Assay Kit employs a recombinant protein derived from single-chain variable fragments (scFv) of an antibody which preferentially binds to the autoantigen in membrane blebs of apoptotic cells. This recombinant protein is fused to protein A allowing visualiztion using fluorescein-conjugated rabbit IgG. Since membrane blebbing occurs only in cells committed to cell death, this assay is specific for cells in the execution stage of apoptosis. Propidium iodide is included in the kit for detection of dead cells, through either a necrotic or apoptotic pathway. The kit is easy to use and can be easily adapted to high throughput screening for therapeutic compounds regulating apoptosis.
Storage : 4°C