Cholesterol Assay Kit

Synonym : Cholesterol/Cholesteryl Ester Quantitation Assay Kit
Stability : 1 year
Description :
Cholesterol Assay provides a simple fluorometric method for the sensitive quantitation of total cholesterol in plasma or serum. In our assay, cholesteryl esters in the sample are first hydrolyzed by cholesterol esterase. All cholesterol, whether previously esterified or existing free in the circulation, is then oxidized by cholesterol oxidase to the corresponding ketone and hydrogen peroxide. ADHP (10-acetyl-3,7-dihydroxyphenoxazine) is utilized as a highly sensitive and stable probe for hydrogen peroxide. Horseradish peroxidase catalyzes the reaction of ADHP with hydrogen peroxide to yield the highly fluorescent product resorufin which can be monitored using excitation wavelengths of 530-580 nm and emission wavelengths of 585-595 nm.
Kit components : 
• Cholesterol Assay Buffer 
• Substrate Mix (lyophilized) 
• Enzyme Mix (lyophilized) 
• Esterase (lyophilized) 
• Cholesterol Standard (2 µg/µl)
Storage : -20°C