PPARα, δ, γ Complete Transcription Factor Assay Kit

Stability : 1 year
Description :
PPARα, δ, γ Complete Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates. A 96 well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the PPAR response element is immobilized onto the bottom of wells of a 96 well plate. PPARs contained in a nuclear extract, bind specifically to the PPAR response element. PPARα, δ, or γ are detected by addition of specific primary antibodies directed against the individual PPARs. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. Cayman’s PPARα, δ, γ Complete Transcription Factor Assay detects PPARα, δ, γ.
storage : -20°C