Description and Properties of Lysozyme

Lysozyme occurs by itself in bulb and beastly tissues and in secretions such as tears, saliva and mucus; it is abnormally abounding in egg whites, which is the primary antecedent for bartering supplies. Hen egg white lysozyme (chick-type or c) was the aboriginal agitator to accept its 2-angstrom clear anatomy bound and has been abundantly studied. Lysozyme is broadly acclimated in bacterial protein extraction. For admittance physique purification, lysozyme is added to abstract corpuscle bits and absolution the admittance bodies. The agitator is calmly alone with added proteins during admixture tag-specific affection ablution of the ambition recombinant protein in basic the admittance body.
Enzymatic function: Catalyzes the hydrolysis of 1,4-β-linkages amid N-acetylmuramic acerbic and N-acetyl-D-glucosamine residues in peptidoglycans and amid N-acetyl-D-glucosamine residues in chitodextrins
Molecular weight: 14.388 kDa
Extinction coefficient: ~26.4 at 280nm
Isoelectric point: pH 11.0
Unit definition: 1 assemblage is that bulk of agitator bare to activate a abatement in absorbance at 450nm of 0.001/min at 25 degrees at pH 6.24 in a 1cm cuvette due to lysis application a ~0.25mg/mL abeyance of Micrococcus luteus.