Effects of Palmatine

A new method using ultrahigh pressure was used to extract palmatine and berberine from the barks of cortex Phellodendri amurensis. The optimal conditions for ultrahigh pressure extraction(UPE) of palmatine and berberine were quantified by HPLC-DAD with standard samples.
Compared with other extraction techniques, such as reflux, ultrasonic, and soxhlet extraction, UPE shows several advantages, such as higher extractionyield, less extraction time, lower extraction temperature and energy consumption. Moreover, UPE may be also a better way to be applied in industry.
The results showed that the UPE method can produce palmatine and berberine with the highest yield. In addition, scanning electron microscope (SEM) images of the plant cells after ultrahigh pressure treatment were obtained to provide visual evidence of the disruption effect.