Heterologous Expression of a Candida molischiana Anth

A recombinant wine aggrandize ache cogent the Candidamolischiana bgln gene encoding a beta-glucosidase/anthocyanase beneath the ascendancy of the Saccharomyces cerevisiae actin gene apostle has been constructed. The agnate protein, BGLN, was mainly amid on the corpuscle wall. BGLN was antiseptic in a individual chromotagraphic step, and altered physicochemical and active backdrop accept been determined.
BGLN showed best action adjoin the bogus substrate p-nitrophenyl beta-D-glucopyranoside. It aswell anatomize salicin, p-nitrophenyl beta-D-xyloside, cellobiose, and arbutin to a bottom extent. Fructose and SO(2) did not affect agitator activity, which was activated by ethanol, while glucose was a able aggressive inhibitor.
The antiseptic BGLN showed a atypical anthocyanase  decolorizing adequacy on red wines. This anthocyanase action was readily empiric during microvinification experiments. However, the physicochemical characteristics of the wines acquired with the recombinant wine aggrandize ache were duplicate from those acquired with the affectionate strain.