What is Arginine Vasopressin EIA Kit?

This Arginine Vasopressin EIA Kit agitator affiliated immunosorbent appraisal applies a address alleged a quantitative sandwich immunoassay. The microtiter bowl provided in this kit has been pre-coated with a monoclonal antibiotic specific for Arginine Vasopressin EIA Kit.
Standards or samples are again added to the microtiter bowl wells and Arginine Vasopressin EIA Kit if present, will bind to the antibiotic pre-coated wells. In adjustment to quantitatively actuate the bulk of ADH/VP/AVP present in the sample, a connected alertness of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ADH/VP/AVP are added to anniversary able-bodied to "sandwich" the Arginine Vasopressin EIA Kit anchored on the plate. The microtiter bowl undergoes incubation, and again the wells are thoroughly done to abolish all absolved components. Next, A and B substrate band-aid is added to anniversary well.
The agitator (HRP) and substrate are accustomed to acknowledge over a abbreviate evolution period.Only those wells that accommodate Arginine Vasopressin EIA Kit and enzyme-conjugated antibiotic will display a change in colour. The enzyme-substrate acknowledgment is concluded by the accession of a sulphuric acerbic band-aid and the colour change is abstinent spectrophotometrically at a amicableness of 450 nm.