5-Lipoxygenase (human recombinant)

Formulation : A solution in 100 mM Tris, pH 8.0, containing 2 mM EGTA
Stability : 6 months
Description : 
Five lipoxygenase (5-LO) is the first committed enzyme in the metabolic pathway leading to the synthesis of the leukotrienes.We examined genomic DNA isolated from 25 normal subjects and 31 patients with asthma (6 of whom had aspirin-sensitive asthma) for mutations in the known transcription factor binding regions and the protein encoding region of the 5-LO gene. A family of mutations in the G1C-rich transcription factor binding region was identified consisting of the deletion of one, deletion of two, or addition of one zinc finger (Sp1/Egr-1) binding sites in the region 176 to 147 bp upstream from the ATG translation start site where there are normally 5 Sp1 binding motifs in tandem.Reporter gene activity directed by any of the mutant forms of the transcription factor binding region was significantly(P,0.05) less effective than the activity driven by the wild type transcription factor binding region.
Purity :  cell lysate 16,000 x g supernatant
Storage : -80°C