Caspase-8 (human recombinant)

Formulation :
A solution in 20 mM Tris-HCl, pH 8.0, containing 500 mM sodium chloride, 20 mM .beta.-mercaptoethanol, 2.5 mM EDTA, 10% sucrose, and 0.1% CHAPS
Stability : 6 months
Description :
Caspase-8 is a member of the interleukin-1 converting enzyme (ICE) family of cysteine proteases. It exists in the cell as an inactive 55 kDa proenzyme. During apoptosis, procaspase-8 is processed at aspartate residues by self-proteolysis and/or cleavage by another caspase. The processed form of caspase-8 consists of large and small subunits which associate to form the active enzyme. Caspase-8 activates downstream caspases (caspase 3, 6, and 7) that cleave key cellular substrates and lead to apoptotic cell death.
Purity : ≥90% (SDS-PAGE), recombinant, expressed in E. coli (C-terminal histidine tagged), buffered aqueous solution, ≥1,000 units/mg protein ;
             ≥95% by SDS-PAGE, Biological Activity: > 5000 units/mg
Appearance : Lyophilized powder
Storage : -80°C