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Last modified
09/05/2018 - 09:08

3α,7α,12α-trihydroxy-5β-cholan-24-oic acid

Product Details

Name Cholic acid
Alias 3α,7α,12α-trihydroxy-5β-cholan-24-oic acid
CAS  No. 81-25-4
Formula C24H40O5
Weight 408.57 g/mol
Usage For research, pharmaceutical intermediates, cosmetics
Appearance Colorless flakes or white crystalline powder

Product Description

This product((3α,7α,12α-trihydroxy-5β- cholan -24-oic acid or Cholic acid )) is found in the particular bile of cattle, sheep and pigs. It is usually a colorless flake or perhaps a white crystalline powder. Several bitterness, after eating it really is somewhat sweet. Mp 198 ° C, specific rotator (c = 0. six, ethanol) is +37 °. 1 g of bile acid is dissolved in 300 ml of ethanol or acetone, 7 ml of glacial acetic acid solution, and is rarely dissolved in water. The monohydrate is really a white flaky amazingly. In 1927, H. Wieland (Germany) studied the composition of bile acids plus won the Nobel Reward in Chemistry.

Identification test
Solubility is extremely hard to dissolve in water; soluble in ethanol. Determined based on the OT-42 method.
The particular melting range is 197 ~ 202 °C. Determined based on conventional methods.
Get ready 0. 02% sample solution with 50% acetic acid solution, take 1 ml, add 1 ml of 1% furfural solution, 6 ml of water and 5 ml of concentrated sulfuric acid. This blend should be converted to rose and then purple in 5 minutes.
Approximately ten mg of the test was taken, 2 falls of benzaldehyde and 3 drops of 3: one sulfuric acid were added, and heated at fifty ° C for 5 min. Add about 10ml of glacial acetic acidity, which should be dark brown.

Content analysis
Approximately four hundred mg of the test after vacuum drying at 140 ° C for 4 h was accurately weighed, put in a 250 ml flask, 20 ml of water and 40 ml of ethanol were added, and the watch glass was covered, and gently heated on a steam bath to dissolve and then cooled down. Add 5 drops of phenolphthalein test solution (TS-167), set it to red with 0. 1 mol/L sodium hydroxide solution and keep it for 15 h while carrying out empty test and make necessary modification. 0. 1 mol/L sodium hydroxide per mL corresponds to 40. 86 mg of cholic acid (C24 H40O5).


Extracted from domestic animals (pig, cattle, sheep, rabbit) bile, an organic acid having a steroid structure, emulsify fat, promote digestion.

(1) It can treat cholecystitis, the lack of bile, intestinal indigestion and gallstones etc.
(2) It can prevent and treat liver diseases.
(3) It is a kind of organic acid and can be used to produce vitamin D2 and D3.



  • Can emulsify fat and improve its function of digestion as a kind of organic acid;
  • Can be applied in the emulator;
  • Can be applied in the research of biochemistry as a kind of medical intermediate;
  • Can be applied in non-ion-modified detergent to extract membrance protein;
  • It is mainly used as medicine raw materials;
  • It is the raw material of cosmetics, and a good surfactant of medicine products;
  • It can be used for additive of animal feed.

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